Naphthalene Archives - One Earth - One Ocean e. V. https://oneearth-oneocean.com/tag/naphthalene/ Sat, 15 Mar 2014 15:16:23 +0000 de-DE hourly 1 https://wordpress.org/?v=6.5.5 https://oneearth-oneocean.com/wp-content/uploads/2022/08/cropped-oeoo-logo-unterzeile-blau-1-32x32.jpg Naphthalene Archives - One Earth - One Ocean e. V. https://oneearth-oneocean.com/tag/naphthalene/ 32 32 Naphthalene biodegradation in temperate and arctic marine microcosms Biodegradation https://oneearth-oneocean.com/research/2014/288/ https://oneearth-oneocean.com/research/2014/288/#respond Sat, 15 Mar 2014 15:16:23 +0000 http://www.oneearth-oneocean.com/kb/?p=288 http://link.springer.com/article/10.1007/s10532-013-9644-3 Andrea Bagi, Daniela M. Pampanin, Anders Lanzén, Torleiv Bilstad, Roald Kommedal Naphthalene biodegradation in temperate and arctic marine microcosms Biodegradation February 2014, Volume 25, Issue 1, pp 111-125 DOI 10.1007/s10532-013-9644-3 Abstract: Naphthalene, the smallest polycyclic aromatic hydrocarbon (PAH), is found in abundance in crude oil, its major source in marine environments. PAH removal occurs via biodegradation, a key process determining their fate in the sea. Adequate estimation of PAH biodegradation rates is essential for environmental risk assessment and response planning using numerical models such as the oil spill contingency and response (OSCAR) model. Using naphthalene as a model compound, biodegradation rate, temperature response and bacterial community composition of seawaters from two climatically different areas (North Sea and Arctic Ocean) were studied and compared. Naphthalene degradation was followed by measuring oxygen consumption in closed bottles using the OxiTop® system. Microbial communities of untreated and naphthalene exposed samples were analysed by polymerase chain reaction denaturing gradient gel electrophoresis (PCR–DGGE) and pyrosequencing. Three times higher naphthalene degradation rate coefficients were observed in arctic seawater samples compared to temperate, at all incubation temperatures. Rate coefficients at in situ temperatures were however, similar (0.048 day−1 for temperate and 0.068 day−1 for arctic). Naphthalene biodegradation rates decreased with similar Q10 ratios (3.3 and 3.5) in both seawaters. Using the temperature compensation method implemented in the OSCAR model, Q10 = 2, biodegradation in arctic seawater was underestimated when calculated from the measured temperate k1 value, showing that temperature difference alone could not predict biodegradation rates adequately. Temperate and arctic untreated seawater communities were different as revealed by pyrosequencing. Geographic origin of seawater affected the community composition of exposed samples. Keywords: Hydrocarbons; Naphthalene; Biodegradation; Q10; Pyrosequencing; DGGE; Seawater

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]]> http://link.springer.com/article/10.1007/s10532-013-9644-3 Andrea Bagi, Daniela M. Pampanin, Anders Lanzén, Torleiv Bilstad, Roald Kommedal Naphthalene biodegradation in temperate and arctic marine microcosms Biodegradation February 2014, Volume 25, Issue 1, pp 111-125 DOI 10.1007/s10532-013-9644-3 Abstract: Naphthalene, the smallest polycyclic aromatic hydrocarbon (PAH), is found in abundance in crude oil, its major source in marine environments. PAH removal occurs via biodegradation, a key process determining their fate in the sea. Adequate estimation of PAH biodegradation rates is essential for environmental risk assessment and response planning using numerical models such as the oil spill contingency and response (OSCAR) model. Using naphthalene as a model compound, biodegradation rate, temperature response and bacterial community composition of seawaters from two climatically different areas (North Sea and Arctic Ocean) were studied and compared. Naphthalene degradation was followed by measuring oxygen consumption in closed bottles using the OxiTop® system. Microbial communities of untreated and naphthalene exposed samples were analysed by polymerase chain reaction denaturing gradient gel electrophoresis (PCR–DGGE) and pyrosequencing. Three times higher naphthalene degradation rate coefficients were observed in arctic seawater samples compared to temperate, at all incubation temperatures. Rate coefficients at in situ temperatures were however, similar (0.048 day−1 for temperate and 0.068 day−1 for arctic). Naphthalene biodegradation rates decreased with similar Q10 ratios (3.3 and 3.5) in both seawaters. Using the temperature compensation method implemented in the OSCAR model, Q10 = 2, biodegradation in arctic seawater was underestimated when calculated from the measured temperate k1 value, showing that temperature difference alone could not predict biodegradation rates adequately. Temperate and arctic untreated seawater communities were different as revealed by pyrosequencing. Geographic origin of seawater affected the community composition of exposed samples. Keywords: Hydrocarbons; Naphthalene; Biodegradation; Q10; Pyrosequencing; DGGE; Seawater

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