enzymatic digestion Archives - One Earth - One Ocean e. V. https://oneearth-oneocean.com/tag/enzymatic-digestion/ Mon, 14 Apr 2014 20:08:55 +0000 de-DE hourly 1 https://wordpress.org/?v=6.5.5 https://oneearth-oneocean.com/wp-content/uploads/2022/08/cropped-oeoo-logo-unterzeile-blau-1-32x32.jpg enzymatic digestion Archives - One Earth - One Ocean e. V. https://oneearth-oneocean.com/tag/enzymatic-digestion/ 32 32 Isolation of microplastics in biota-rich seawater samples and marine organisms https://oneearth-oneocean.com/research/2014/isolation-of-microplastics-in-biota-rich-seawater-samples-and-marine-organisms/ https://oneearth-oneocean.com/research/2014/isolation-of-microplastics-in-biota-rich-seawater-samples-and-marine-organisms/#respond Mon, 14 Apr 2014 20:08:55 +0000 http://www.oneearth-oneocean.com/kb/?p=466 http://www.nature.com/srep/2014/140331/srep04528/full/srep04528.html Matthew Cole, Hannah Webb, Pennie K. Lindeque, Elaine S. Fileman, Claudia Halsband & Tamara S. Galloway Isolation of microplastics in biota-rich seawater samples and marine organisms Scientific Reports 4, Article number: 4528, doi:10.1038/srep04528 Abstract Microplastic litter is a pervasive pollutant present in aquatic systems across the globe. A range of marine organisms have the capacity to ingest microplastics, resulting in adverse health effects. Developing methods to accurately quantify microplastics in productive marine waters, and those internalized by marine organisms, is of growing importance. Here we investigate the efficacy of using acid, alkaline and enzymatic digestion techniques in mineralizing biological material from marine surface trawls to reveal any microplastics present. Our optimized enzymatic protocol can digest >97% (by weight) of the material present in plankton-rich seawater samples without destroying any microplastic debris present. In applying the method to replicate marine samples from the western English Channel, we identified 0.27 microplastics m?3. The protocol was further used to extract microplastics ingested by marine zooplankton under laboratory conditions. Our findings illustrate that enzymatic digestion can aid the detection of microplastic debris within seawater samples and marine biota. http://www.nature.com/srep/2014/140331/srep04528/pdf/srep04528.pdf http://www.nature.com/srep/2014/140331/srep04528/extref/srep04528-s1.doc

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]]> http://www.nature.com/srep/2014/140331/srep04528/full/srep04528.html Matthew Cole, Hannah Webb, Pennie K. Lindeque, Elaine S. Fileman, Claudia Halsband & Tamara S. Galloway Isolation of microplastics in biota-rich seawater samples and marine organisms Scientific Reports 4, Article number: 4528, doi:10.1038/srep04528 Abstract Microplastic litter is a pervasive pollutant present in aquatic systems across the globe. A range of marine organisms have the capacity to ingest microplastics, resulting in adverse health effects. Developing methods to accurately quantify microplastics in productive marine waters, and those internalized by marine organisms, is of growing importance. Here we investigate the efficacy of using acid, alkaline and enzymatic digestion techniques in mineralizing biological material from marine surface trawls to reveal any microplastics present. Our optimized enzymatic protocol can digest >97% (by weight) of the material present in plankton-rich seawater samples without destroying any microplastic debris present. In applying the method to replicate marine samples from the western English Channel, we identified 0.27 microplastics m?3. The protocol was further used to extract microplastics ingested by marine zooplankton under laboratory conditions. Our findings illustrate that enzymatic digestion can aid the detection of microplastic debris within seawater samples and marine biota. http://www.nature.com/srep/2014/140331/srep04528/pdf/srep04528.pdf http://www.nature.com/srep/2014/140331/srep04528/extref/srep04528-s1.doc

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