Bacillus subtilis H1584 Archives - One Earth - One Ocean e. V. https://oneearth-oneocean.com/tag/bacillus-subtilis-h1584/ Sun, 22 Dec 2013 20:04:37 +0000 de-DE hourly 1 https://wordpress.org/?v=6.5.5 https://oneearth-oneocean.com/wp-content/uploads/2022/08/cropped-oeoo-logo-unterzeile-blau-1-32x32.jpg Bacillus subtilis H1584 Archives - One Earth - One Ocean e. V. https://oneearth-oneocean.com/tag/bacillus-subtilis-h1584/ 32 32 Biodegradation of low-density polyethylene by marine bacteria from pelagic waters, Arabian Sea, India https://oneearth-oneocean.com/2013/2013/biodegradation-of-low-density-polyethylene-by-marine-bacteria-from-pelagic-waters-arabian-sea-india-2/ https://oneearth-oneocean.com/2013/2013/biodegradation-of-low-density-polyethylene-by-marine-bacteria-from-pelagic-waters-arabian-sea-india-2/#respond Sun, 22 Dec 2013 20:04:37 +0000 http://www.oneearth-oneocean.com/kb/?p=122 Kumar Harshvardhan, Bhavanath Jha, Biodegradation of low-density polyethylene by marine bacteria from pelagic waters, Arabian Sea, India, Marine Pollution Bulletin, Available online 7 November 2013, ISSN 0025-326X, http://dx.doi.org/10.1016/j.marpolbul.2013.10.025. (http://www.sciencedirect.com/science/article/pii/S0025326X13006462) Abstract: Sixty marine bacteria isolated from pelagic waters were screened for their ability to degrade low-density polyethylene; among them, three were positive and able to grow in a medium containing polythene as the sole carbon source. The positive isolates were identified as Kocuria palustris M16, Bacillus pumilus M27 and Bacillus subtilis H1584 based on the 16S rRNA gene sequence homology. The weight loss of polyethylene was 1%, 1.5% and 1.75% after 30 days of incubation with the M16, M27 and H1584 isolates, respectively. The maximum (32%) cell surface hydrophobicity was observed in M16, followed by the H1584 and M27 isolates. The viability of the isolates growing on the polyethylene surface was confirmed using a triphenyltetrazolium chloride reduction test. The viability was also correlated with a concomitant increase in the protein density of the biomass. Polyethylene biodegradation was further confirmed by an increase in the Keto Carbonyl Bond Index, the Ester Carbonyl Bond Index and the Vinyl Bond Index, which were calculated from FT-IR spectra.

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]]> Kumar Harshvardhan, Bhavanath Jha, Biodegradation of low-density polyethylene by marine bacteria from pelagic waters, Arabian Sea, India, Marine Pollution Bulletin, Available online 7 November 2013, ISSN 0025-326X, http://dx.doi.org/10.1016/j.marpolbul.2013.10.025. (http://www.sciencedirect.com/science/article/pii/S0025326X13006462) Abstract: Sixty marine bacteria isolated from pelagic waters were screened for their ability to degrade low-density polyethylene; among them, three were positive and able to grow in a medium containing polythene as the sole carbon source. The positive isolates were identified as Kocuria palustris M16, Bacillus pumilus M27 and Bacillus subtilis H1584 based on the 16S rRNA gene sequence homology. The weight loss of polyethylene was 1%, 1.5% and 1.75% after 30 days of incubation with the M16, M27 and H1584 isolates, respectively. The maximum (32%) cell surface hydrophobicity was observed in M16, followed by the H1584 and M27 isolates. The viability of the isolates growing on the polyethylene surface was confirmed using a triphenyltetrazolium chloride reduction test. The viability was also correlated with a concomitant increase in the protein density of the biomass. Polyethylene biodegradation was further confirmed by an increase in the Keto Carbonyl Bond Index, the Ester Carbonyl Bond Index and the Vinyl Bond Index, which were calculated from FT-IR spectra.

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