{"id":32265,"date":"2013-12-22T22:04:37","date_gmt":"2013-12-22T21:04:37","guid":{"rendered":"https:\/\/oneearth-oneocean.com\/2013\/12\/22\/biodegradation-of-low-density-polyethylene-by-marine-bacteria-from-pelagic-waters-arabian-sea-india-2\/"},"modified":"2022-12-20T22:43:29","modified_gmt":"2022-12-20T21:43:29","slug":"biodegradation-of-low-density-polyethylene-by-marine-bacteria-from-pelagic-waters-arabian-sea-india-2","status":"publish","type":"post","link":"https:\/\/oneearth-oneocean.com\/en\/research-en\/2013\/biodegradation-of-low-density-polyethylene-by-marine-bacteria-from-pelagic-waters-arabian-sea-india-2\/","title":{"rendered":"Biodegradation of low-density  polyethylene by marine bacteria from pelagic waters, Arabian Sea, India"},"content":{"rendered":"<pre>Kumar Harshvardhan, Bhavanath Jha, Biodegradation of low-density \npolyethylene by marine bacteria from pelagic waters, Arabian Sea, India, \nMarine Pollution Bulletin, Available online 7 November 2013, ISSN \n0025-326X, <a href=\"http:\/\/dx.doi.org\/10.1016\/j.marpolbul.2013.10.025\" target=\"_blank\" rel=\"noopener\">http:\/\/dx.doi.org\/10.1016\/j.marpolbul.2013.10.025<\/a>.\n(<a href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0025326X13006462\" target=\"_blank\" rel=\"noopener\">http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0025326X13006462<\/a>)\nAbstract: Sixty marine bacteria isolated from pelagic waters were \nscreened for their ability to degrade low-density polyethylene; among \nthem, three were positive and able to grow in a medium containing \npolythene as the sole carbon source. The positive isolates were \nidentified as Kocuria palustris M16, Bacillus pumilus M27 and Bacillus \nsubtilis H1584 based on the 16S rRNA gene sequence homology. The weight \nloss of polyethylene was 1%, 1.5% and 1.75% after 30 days of incubation \nwith the M16, M27 and H1584 isolates, respectively. The maximum (32%) \ncell surface hydrophobicity was observed in M16, followed by the H1584 \nand M27 isolates. The viability of the isolates growing on the \npolyethylene surface was confirmed using a triphenyltetrazolium chloride \nreduction test. The viability was also correlated with a concomitant \nincrease in the protein density of the biomass. Polyethylene \nbiodegradation was further confirmed by an increase in the Keto Carbonyl \nBond Index, the Ester Carbonyl Bond Index and the Vinyl Bond Index, \nwhich were calculated from FT-IR spectra.<\/pre>\n","protected":false},"excerpt":{"rendered":"<p>Kumar Harshvardhan, Bhavanath Jha, Biodegradation of low-density polyethylene by marine bacteria from pelagic waters, Arabian Sea, India, Marine Pollution Bulletin, Available online 7 November 2013, ISSN 0025-326X, http:\/\/dx.doi.org\/10.1016\/j.marpolbul.2013.10.025. (http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0025326X13006462) Abstract: Sixty marine bacteria isolated from pelagic waters were screened for their ability to degrade low-density polyethylene; among them, three were positive and able to grow in a medium containing polythene as the sole carbon source. The positive isolates were identified as Kocuria palustris M16, Bacillus pumilus M27 and Bacillus subtilis H1584 based on the 16S rRNA gene sequence homology. The weight loss of polyethylene was 1%, 1.5% and 1.75% after 30 days of incubation with the M16, M27 and H1584 isolates, respectively. The maximum (32%) cell surface hydrophobicity was observed in M16, followed by the H1584 and M27 isolates. The viability of the isolates growing on the polyethylene surface was confirmed using a triphenyltetrazolium chloride reduction test. The viability was also correlated with a concomitant increase in the protein density of the biomass. Polyethylene biodegradation was further confirmed by an increase in the Keto Carbonyl Bond Index, the Ester Carbonyl Bond Index and the Vinyl Bond Index, which were calculated from FT-IR spectra.<\/p>\n","protected":false},"author":7,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3649,3613,3941,3423],"tags":[4010,4011,4012,3460,4013,4014,2290],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Biodegradation of low-density polyethylene by marine bacteria from pelagic waters, Arabian Sea, India - One Earth - One Ocean e. V.<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/oneearth-oneocean.com\/en\/research-en\/2013\/biodegradation-of-low-density-polyethylene-by-marine-bacteria-from-pelagic-waters-arabian-sea-india-2\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Biodegradation of low-density polyethylene by marine bacteria from pelagic waters, Arabian Sea, India - One Earth - One Ocean e. V.\" \/>\n<meta property=\"og:description\" content=\"Kumar Harshvardhan, Bhavanath Jha, Biodegradation of low-density polyethylene by marine bacteria from pelagic waters, Arabian Sea, India, Marine Pollution Bulletin, Available online 7 November 2013, ISSN 0025-326X, http:\/\/dx.doi.org\/10.1016\/j.marpolbul.2013.10.025. (http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0025326X13006462) Abstract: Sixty marine bacteria isolated from pelagic waters were screened for their ability to degrade low-density polyethylene; among them, three were positive and able to grow in a medium containing polythene as the sole carbon source. The positive isolates were identified as Kocuria palustris M16, Bacillus pumilus M27 and Bacillus subtilis H1584 based on the 16S rRNA gene sequence homology. The weight loss of polyethylene was 1%, 1.5% and 1.75% after 30 days of incubation with the M16, M27 and H1584 isolates, respectively. The maximum (32%) cell surface hydrophobicity was observed in M16, followed by the H1584 and M27 isolates. The viability of the isolates growing on the polyethylene surface was confirmed using a triphenyltetrazolium chloride reduction test. The viability was also correlated with a concomitant increase in the protein density of the biomass. Polyethylene biodegradation was further confirmed by an increase in the Keto Carbonyl Bond Index, the Ester Carbonyl Bond Index and the Vinyl Bond Index, which were calculated from FT-IR spectra.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/oneearth-oneocean.com\/en\/research-en\/2013\/biodegradation-of-low-density-polyethylene-by-marine-bacteria-from-pelagic-waters-arabian-sea-india-2\/\" \/>\n<meta property=\"og:site_name\" content=\"One Earth - One Ocean e. 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(http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0025326X13006462) Abstract: Sixty marine bacteria isolated from pelagic waters were screened for their ability to degrade low-density polyethylene; among them, three were positive and able to grow in a medium containing polythene as the sole carbon source. The positive isolates were identified as Kocuria palustris M16, Bacillus pumilus M27 and Bacillus subtilis H1584 based on the 16S rRNA gene sequence homology. The weight loss of polyethylene was 1%, 1.5% and 1.75% after 30 days of incubation with the M16, M27 and H1584 isolates, respectively. The maximum (32%) cell surface hydrophobicity was observed in M16, followed by the H1584 and M27 isolates. The viability of the isolates growing on the polyethylene surface was confirmed using a triphenyltetrazolium chloride reduction test. The viability was also correlated with a concomitant increase in the protein density of the biomass. 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