{"id":31778,"date":"2014-04-14T22:08:55","date_gmt":"2014-04-14T20:08:55","guid":{"rendered":"https:\/\/oneearth-oneocean.com\/2014\/04\/14\/isolation-of-microplastics-in-biota-rich-seawater-samples-and-marine-organisms\/"},"modified":"2014-04-14T22:08:55","modified_gmt":"2014-04-14T20:08:55","slug":"isolation-of-microplastics-in-biota-rich-seawater-samples-and-marine-organisms","status":"publish","type":"post","link":"https:\/\/oneearth-oneocean.com\/en\/atlantic-en\/2014\/isolation-of-microplastics-in-biota-rich-seawater-samples-and-marine-organisms\/","title":{"rendered":"Isolation of microplastics in biota-rich seawater samples and marine organisms"},"content":{"rendered":"
http:\/\/www.nature.com\/srep\/2014\/140331\/srep04528\/full\/srep04528.html<\/a>\n\nMatthew Cole, Hannah Webb, Pennie K. Lindeque, Elaine S. Fileman,\nClaudia Halsband & Tamara S. Galloway\nIsolation of microplastics in biota-rich seawater samples and marine\norganisms\nScientific Reports 4, Article number: 4528, doi:10.1038\/srep04528\n\nAbstract\nMicroplastic litter is a pervasive pollutant present in aquatic systems\nacross the globe. A range of marine organisms have the capacity to\ningest microplastics, resulting in adverse health effects. Developing\nmethods to accurately quantify microplastics in productive marine\nwaters, and those internalized by marine organisms, is of growing\nimportance. Here we investigate the efficacy of using acid, alkaline and\nenzymatic digestion techniques in mineralizing biological material from\nmarine surface trawls to reveal any microplastics present. Our optimized\nenzymatic protocol can digest >97% (by weight) of the material present\nin plankton-rich seawater samples without destroying any microplastic\ndebris present. In applying the method to replicate marine samples from\nthe western English Channel, we identified 0.27 microplastics m?3. The\nprotocol was further used to extract microplastics ingested by marine\nzooplankton under laboratory conditions. Our findings illustrate that\nenzymatic digestion can aid the detection of microplastic debris within\nseawater samples and marine biota.\n\nhttp:\/\/www.nature.com\/srep\/2014\/140331\/srep04528\/pdf\/srep04528.pdf<\/a>\n\nhttp:\/\/www.nature.com\/srep\/2014\/140331\/srep04528\/extref\/srep04528-s1.doc<\/a><\/pre>\n","protected":false},"excerpt":{"rendered":"
http:\/\/www.nature.com\/srep\/2014\/140331\/srep04528\/full\/srep04528.html Matthew Cole, Hannah Webb, Pennie K. Lindeque, Elaine S. Fileman, Claudia Halsband & Tamara S. Galloway Isolation of microplastics in biota-rich seawater samples and marine organisms Scientific Reports 4, Article number: 4528, doi:10.1038\/srep04528 Abstract Microplastic litter is a pervasive pollutant present in aquatic systems across the globe. A range of marine organisms have the capacity to ingest microplastics, resulting in adverse health effects. Developing methods to accurately quantify microplastics in productive marine waters, and those internalized by marine organisms, is of growing importance. Here we investigate the efficacy of using acid, alkaline and enzymatic digestion techniques in mineralizing biological material from marine surface trawls to reveal any microplastics present. Our optimized enzymatic protocol can digest >97% (by weight) of the material present in plankton-rich seawater samples without destroying any microplastic debris present. In applying the method to replicate marine samples from the western English Channel, we identified 0.27 microplastics m?3. The protocol was further used to extract microplastics ingested by marine zooplankton under laboratory conditions. Our findings illustrate that enzymatic digestion can aid the detection of microplastic debris within seawater samples and marine biota. http:\/\/www.nature.com\/srep\/2014\/140331\/srep04528\/pdf\/srep04528.pdf http:\/\/www.nature.com\/srep\/2014\/140331\/srep04528\/extref\/srep04528-s1.doc<\/p>\n","protected":false},"author":7,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3504,3415,3423],"tags":[3667,1585,1445],"yoast_head":"\nIsolation of microplastics in biota-rich seawater samples and marine organisms - One Earth - One Ocean e. V.<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/oneearth-oneocean.com\/en\/atlantic-en\/2014\/isolation-of-microplastics-in-biota-rich-seawater-samples-and-marine-organisms\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Isolation of microplastics in biota-rich seawater samples and marine organisms - One Earth - One Ocean e. V.\" \/>\n<meta property=\"og:description\" content=\"http:\/\/www.nature.com\/srep\/2014\/140331\/srep04528\/full\/srep04528.html Matthew Cole, Hannah Webb, Pennie K. Lindeque, Elaine S. Fileman, Claudia Halsband & Tamara S. Galloway Isolation of microplastics in biota-rich seawater samples and marine organisms Scientific Reports 4, Article number: 4528, doi:10.1038\/srep04528 Abstract Microplastic litter is a pervasive pollutant present in aquatic systems across the globe. A range of marine organisms have the capacity to ingest microplastics, resulting in adverse health effects. Developing methods to accurately quantify microplastics in productive marine waters, and those internalized by marine organisms, is of growing importance. Here we investigate the efficacy of using acid, alkaline and enzymatic digestion techniques in mineralizing biological material from marine surface trawls to reveal any microplastics present. Our optimized enzymatic protocol can digest >97% (by weight) of the material present in plankton-rich seawater samples without destroying any microplastic debris present. In applying the method to replicate marine samples from the western English Channel, we identified 0.27 microplastics m?3. The protocol was further used to extract microplastics ingested by marine zooplankton under laboratory conditions. Our findings illustrate that enzymatic digestion can aid the detection of microplastic debris within seawater samples and marine biota. http:\/\/www.nature.com\/srep\/2014\/140331\/srep04528\/pdf\/srep04528.pdf http:\/\/www.nature.com\/srep\/2014\/140331\/srep04528\/extref\/srep04528-s1.doc\" \/>\n<meta property=\"og:url\" content=\"https:\/\/oneearth-oneocean.com\/en\/atlantic-en\/2014\/isolation-of-microplastics-in-biota-rich-seawater-samples-and-marine-organisms\/\" \/>\n<meta property=\"og:site_name\" content=\"One Earth - One Ocean e. 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A range of marine organisms have the capacity to ingest microplastics, resulting in adverse health effects. Developing methods to accurately quantify microplastics in productive marine waters, and those internalized by marine organisms, is of growing importance. Here we investigate the efficacy of using acid, alkaline and enzymatic digestion techniques in mineralizing biological material from marine surface trawls to reveal any microplastics present. Our optimized enzymatic protocol can digest >97% (by weight) of the material present in plankton-rich seawater samples without destroying any microplastic debris present. In applying the method to replicate marine samples from the western English Channel, we identified 0.27 microplastics m?3. The protocol was further used to extract microplastics ingested by marine zooplankton under laboratory conditions. 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