Influence of microplastics on the accumulation and chronic toxic effects of cadmium in zebrafish (Danio rerio),

Chemosphere,
2018,
ISSN 0045-6535,

Abstract:

As the accumulation of microplastics (MPs) in the environment
continues to rise, more concerns focus on the health risk of combined
exposure to MPs and other contaminants. The aim of this study is to
investigate the influences of MPs on the tissue-accumulation of cadmium
(Cd) in zebrafish and explore the related chronic toxic effects induced
by combined exposure of Cd and MPs. After co-exposure to MPs and Cd for
3 weeks, 20 and 200 μg/L MPs increased the accumulation of Cd in
zebrafish livers (46% and 184%), guts (10% and 25%) and gills (9% and
46%). The Cd accumulation was gill > gut > liver. Comprehensive analyzes
of biochemical biomarkers, histopathological observation and functional
gene expression firstly demonstrated that the presence of MPs enhanced
the toxicity of Cd on zebrafish and the combined exposure caused
oxidative damage and inflammation in zebrafish tissues. Collectively,
our results highlight the chronic effects of combined exposure to MPs
and heavy metals.

https://drive.google.com/open?id=19QNBzSwS5Fa24OhiUuVA6i0mES68Q5vh
https://doi.org/10.1016/j.chemosphere.2018.03.145.(http://www.sciencedirect.com/science/article/pii/S0045653518305629)

The post Influence of microplastics on the accumulation and chronic toxic effects of cadmium in zebrafish (Danio rerio) appeared first on One Earth - One Ocean e. V..

Abstract:

As the accumulation of microplastics (MPs) in the environment
continues to rise, more concerns focus on the health risk of combined
exposure to MPs and other contaminants. The aim of this study is to
investigate the influences of MPs on the tissue-accumulation of cadmium
(Cd) in zebrafish and explore the related chronic toxic effects induced
by combined exposure of Cd and MPs. After co-exposure to MPs and Cd for
3 weeks, 20 and 200 μg/L MPs increased the accumulation of Cd in
zebrafish livers (46% and 184%), guts (10% and 25%) and gills (9% and
46%). The Cd accumulation was gill > gut > liver. Comprehensive analyzes
of biochemical biomarkers, histopathological observation and functional
gene expression firstly demonstrated that the presence of MPs enhanced
the toxicity of Cd on zebrafish and the combined exposure caused
oxidative damage and inflammation in zebrafish tissues. Collectively,
our results highlight the chronic effects of combined exposure to MPs
and heavy metals.
Keywords: Microplastics; Heavy metal; Combined exposure; Chronic
toxicity; Zebrafish

https://drive.google.com/open?id=19QNBzSwS5Fa24OhiUuVA6i0mES68Q5vh
https://doi.org/10.1016/j.chemosphere.2018.03.145.(http://www.sciencedirect.com/science/article/pii/S0045653518305629)

The post Influence of microplastics on the accumulation and chronic toxic effects of cadmium in zebrafish (Danio rerio) appeared first on One Earth - One Ocean e. V..

Influence of microplastics on the accumulation and chronic toxic effects of cadmium in zebrafish (Danio rerio),
Chemosphere,

Volume 202,
2018,
Pages 514-520,
ISSN 0045-6535,

Abstract:

As the accumulation of microplastics (MPs) in the environment continues to rise, more concerns focus on the health risk of combinedexposure to MPs and other contaminants.
The aim of this study is to investigate the influences of MPs on the tissue-accumulation of cadmium
(Cd) in zebrafish and explore the related chronic toxic effects induced by combined exposure of Cd and MPs.
After co-exposure to MPs and Cd for 3 weeks, 20 and 200 μg/L MPs increased the accumulation of Cd in
zebrafish livers (46% and 184%), guts (10% and 25%) and gills (9% and 46%).
The Cd accumulation was gill > gut > liver. Comprehensive analyzesof biochemical biomarkers, histopathological observation and functionalgene expression firstly demonstrated that the presence of MPs enhanced
the toxicity of Cd on zebrafish and the combined exposure caused oxidative damage and inflammation in zebrafish tissues.
Collectively, our results highlight the chronic effects of combined exposure to MPs and heavy metals.

https://drive.google.com/open?id=19QNBzSwS5Fa24OhiUuVA6i0mES68Q5vh
https://doi.org/10.1016/j.chemosphere.2018.03.145.(http://www.sciencedirect.com/science/article/pii/S0045653518305629)

The post Influence of microplastics on the accumulation and chronic toxic effects of cadmium in zebrafish (Danio rerio), Chemosphere, appeared first on One Earth - One Ocean e. V..

Environmental Pollution, Volume 235, 2018, Pages 322-329, ISSN 0269-7491,

Abstract:

Microplastic (MP) are environmental pollutants and have the
potential to cause varying degrees of aquatic toxicity. In this study,
the effects on gut microbiota of adult male zebrafish exposed for 14
days to 100 and 1000 μg/L of two sizes of polystyrene MP were evaluated.
Both 0.5 and 50 μm-diameter spherical polystyrene MP increased the
volume of mucus in the gut at a concentration of 1000 μg/L (about
1.456 × 1010 particles/L for 0.5 μm and 1.456 × 104 particles/L for
50 μm). At the phylum level, the abundance of Bacteroidetes and
Proteobacteria decreased significantly and the abundance of Firmicutes
increased significantly in the gut after 14-day exposure to 1000 μg/L of
both sizes of polystyrene MP. In addition, high throughput sequencing of
the 16S rRNA gene V3-V4 region revealed a significant change in the
richness and diversity of microbiota in the gut of polystyrene
MP-exposed zebrafish. A more in depth analysis, at the genus level,
revealed that a total of 29 gut microbes identified by operational
taxonomic unit (OTU) analysis were significantly changed in both 0.5 and
50 μm-diameter polystyrene MP-treated groups. Moreover, it was observed
that 0.5 μm polystyrene MP not only increased mRNA levels of IL1α, IL1β
and IFN but also their protein levels in the gut, indicating that
inflammation occurred after polystyrene MP exposure. Our findings
suggest that polystyrene MP could induce microbiota dysbiosis and
inflammation in the gut of adult zebrafish.

https://drive.google.com/open?id=1q4N4madUKJasGvOfzEK4nyvPVOv38M-i

https://doi.org/10.1016/j.envpol.2017.12.088.(http://www.sciencedirect.com/science/article/pii/S0269749117315464)

The post Polystyrene microplastics induce microbiota dysbiosis and inflammation in the gut of adult zebrafish appeared first on One Earth - One Ocean e. V..

Polystyrene microplastics induce microbiota dysbiosis and inflammation
in the gut of adult zebrafish,
Environmental Pollution, Volume 235, 2018, Pages 322-329, ISSN 0269-7491,

Abstract:

Microplastic (MP) are environmental pollutants and have the potential to cause varying degrees of aquatic toxicity. In this study, the effects on gut microbiota of adult male zebrafish exposed for 14 days to 100 and 1000 μg/L of two sizes of polystyrene MP were evaluated.
Both 0.5 and 50 μm-diameter spherical polystyrene MP increased the volume of mucus in the gut at a concentration of 1000 μg/L (about 1.456 × 1010 particles/L for 0.5 μm and 1.456 × 104 particles/L for 50 μm). At the phylum level, the abundance of Bacteroidetes and Proteobacteria decreased significantly and the abundance of Firmicutes increased significantly in the gut after 14-day exposure to 1000 μg/L of both sizes of polystyrene MP. In addition, high throughput sequencing of the 16S rRNA gene V3-V4 region revealed a significant change in the richness and diversity of microbiota in the gut of polystyrene MP-exposed zebrafish.
A more in depth analysis, at the genus level, revealed that a total of 29 gut microbes identified by operational taxonomic unit (OTU) analysis were significantly changed in both 0.5 and 50 μm-diameter polystyrene MP-treated groups. Moreover, it was observed
that 0.5 μm polystyrene MP not only increased mRNA levels of IL1α, IL1β and IFN but also their protein levels in the gut, indicating that inflammation occurred after polystyrene MP exposure. Our findings suggest that polystyrene MP could induce microbiota dysbiosis and inflammation in the gut of adult zebrafish.

https://drive.google.com/open?id=1q4N4madUKJasGvOfzEK4nyvPVOv38M-i
https://doi.org/10.1016/j.envpol.2017.12.088.(http://www.sciencedirect.com/science/article/pii/S0269749117315464)

The post Polystyrene microplastics induce microbiota dysbiosis and inflammation in the gut of adult zebrafish appeared first on One Earth - One Ocean e. V..